§113.47. Detection of extraneous viruses by the fluorescent antibody technique. — Inbound Citations

9 C.F.R. § 113.47

Statutory Authority

Cited by 5 regulations in release Current.

9 C.F.R. § 113.51(c)(1)(i)

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(i) Monolayers of avian origin shall be maintained for at least 14 days and shall be subcultured at least once during the maintenance period. All but the last subculture shall result in a new monolayer of at least 75 cm 2. The last subculture shall meet the minimum area requirement specified in §§ 113.46 and 113.47.
9 C.F.R. § 113.52(e)(2)

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(2) Cells shall be subcultured at least two times during the maintenance period. All but the last subculture shall result in at least one new monolayer of at least 75 cm 2. The last subculture shall meet the minimum area requirement specified in §§ 113.46 and 113.47 and paragraph (f) of this section.
9 C.F.R. § 113.53(c)(4)

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(4) Cells shall be subcultured at least two times during the maintenance period. All but the last subculture shall result in at least one new monolayer of at least 75 cm 2. The last subculture shall meet the minimum area requirements specified in §§ 113.46 and 113.47.
9 C.F.R. § 113.55(d)

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(d) Cells shall be subcultured at least once during the maintenance period. All but the last subculture shall result in at least one new monolayer at least 75 cm 2. The last subculture shall meet the minimum area requirement specified in §§ 113.46 and 113.47.

9 C.F.R. § 113.53(d)(3)

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(3) At the end of the 14-day maintenance period, and 4 to 7 days after the last subculturing, monolayers shall be tested for the presence of porcine parvovirus by the fluorescent antibody technique as prescribed in § 113.47(c).