§113.34. Detection of hemagglutinating viruses. — Inbound Citations
9 C.F.R. § 113.34
Statutory Authority
Cited by 4 regulations in release Current.
Citations to 9 U.S.C. § 113.34 as a whole
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(iii) as prescribed in § 113.34.
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(iii) Hemagglutinating viruses as prescribed in § 113.34.
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(i) The Newcastle disease virus fraction of combined Newcastle-Bronchitis Vaccines shall be neutralized prior to titration of the bronchitis virus fraction. Equal parts of heat-inactivated Newcastle disease antiserum shall be mixed with each appropriate serial ten-fold dilution of the vaccine. After inactivation, embryos shall be injected with 0.2 ml each and results calculated as a 0.1 ml dose to allow for serum dilution of the vaccine. The allantoic fluids, tested as prescribed in § 113.34 shall not show hemagglutinating activity in the lowest dilution used in the titration.
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