§113.32. Detection of Brucella contamination.

One ml of the minced tissue used as the source of cells or 1 ml of the extract of the tissue prior to the addition of antibiotics, diluent and stabilizer, shall be inoculated onto each of three tryptose agar plates and incubated in a 10 percent CO2 atmosphere at a temperature of 35-37 °C for at least 7 days.

If colonies are identified as Brucella, the biological product is unsatisfactory.

If colonies suspicious of Brucella are observed but cannot be identified as a Brucella species, either