---
kind: "section"
citation: "9 C.F.R. § 113.32"
title: "9"
number: "113.32"
heading: "Detection of Brucella contamination."
url: "https://uscodex.org/cfr/9/113.32"
---

# §113.32. Detection of Brucella contamination.


The test for detection of Brucella contamination provided in this section shall be conducted when such a test is prescribed in an applicable Standard Requirement or in a filed Outline of Production for the product.

- (a) One ml of the minced tissue used as the source of cells or 1 ml of the extract of the tissue prior to the addition of antibiotics, diluent and stabilizer, shall be inoculated onto each of three tryptose agar plates and incubated in a 10 percent CO2 atmosphere at a temperature of 35-37 °C for at least 7 days.
- (b) If colonies are identified as Brucella, the biological product is unsatisfactory.
- (c) If colonies suspicious of Brucella are observed but cannot be identified as a Brucella species, either
  - (1) The biological product shall be regarded as unsatisfactory and destroyed; or
  - (2) Further subculture or other procedures shall be carried out until a positive identification can be made.

## Notes

### Amendments

[38 FR 29888, Oct. 30, 1973]

### Authority

Authority: 21 U.S.C. 151-159; 7 CFR 2.22, 2.80, and 371.4.

### Source

Source: 34 FR 18004, Nov. 7, 1969, unless otherwise noted.

### Amendments

[38 FR 29888, Oct. 30, 1973]