---
kind: "section"
citation: "40 C.F.R. § 180.1027"
title: "40"
number: "180.1027"
heading: "Nuclear polyhedrosis virus of Heliothis zea; exemption from the requirement of a tolerance."
url: "https://uscodex.org/cfr/40/180.1027"
---

# §180.1027. Nuclear polyhedrosis virus of Heliothis zea; exemption from the requirement of a tolerance.

- (a) For the purposes of this section, the viral insecticide must be produced with an unaltered and unadulterated inoculum of the single-embedded Heliothis zea nuclear polyhedrosis virus (HzSNPV). The identity of the seed virus must be assured by periodic checks.
- (b) Each lot of active ingredient of the viral insecticide shall have the following specifications:
  - (1) The level of extraneous bacterial contamination of the final unformulated viral insecticide should not exceed 10 7 colonies per gram as determined by an aerobic plate on trypticase soy agar.
  - (2) Human pathogens, e.g., Salmonella, Shigella, or Vibrio, must be absent.
  - (3) Safety to mice as determined by an intraperitoneal injection study must be demonstrated.
  - (4) Identity of the viral product, as determined by the most sensitive and standardized analytical technique, e.g., restriction endonuclease and/or SDS-PAGE analysis, must be demonstrated.
- (c) Exemptions from the requirement of a tolerance are established for the residues of the microbial insecticide Heliothis zea NPV, as specified in paragraphs [(a)](#a) and [(b)](#b) of this section, in or on all agricultural commodities.

## Notes

### Amendments

[60 FR 42460, Aug. 16, 1995, as amended at 74 FR 26534, June 3, 2009]

### Authority

Authority: 21 U.S.C. 321(q), 346a and 371.

### Source

Source: 36 FR 22540, Nov. 25, 1971, unless otherwise noted.

### Amendments

[60 FR 42460, Aug. 16, 1995, as amended at 74 FR 26534, June 3, 2009]