---
kind: "section"
citation: "37 C.F.R. § 1.824"
title: "37"
number: "1.824"
heading: "Form and format for a nucleotide and/or amino acid sequence submission as an ASCII plain text file."
url: "https://uscodex.org/cfr/37/1.824"
---

# §1.824. Form and format for a nucleotide and/or amino acid sequence submission as an ASCII plain text file.

- (a) A “Sequence Listing” under [§ 1.821(c)(1)](/cfr/37/1.821.md?p=c-1) and the CRF required by [§ 1.821(e)](/cfr/37/1.821.md?p=e) submitted as an ASCII plain text file may be created by any means, such as text editors, nucleotide/amino acid sequence editors, or other custom computer programs; however, the ASCII plain text file must conform to the following requirements:
  - (1) **Must have the following compatibilities—**
    - (i) **Computer compatibility—** PC or Mac®; and
    - (ii) **Operating system compatibility—** MS-DOS®, MS-Windows®, Mac OS®, or Unix®/Linux®.
  - (2) **Must be in ASCII plain text, where—**
    - (i) All printable characters (including the space character) are permitted; and
    - (ii) **No nonprintable (ASCII control) characters are permitted, except ASCII CRLF or LF as line terminators.**
  - (3) Must be named as *.txt, where “*” is one character or a combination of characters limited to upper- or lowercase letters, numbers, hyphens, and underscores and does not exceed 60 characters in total, excluding the extension. No spaces or other types of characters are permitted in the file name.
  - (4) **Must contain no more than 74 printable characters in each line.**
  - (5) Pagination is not permitted; the ASCII plain text file must be one continuous file, with no “hard page break” codes and no page numbering.
- (b) The ASCII plain text file must contain a copy of a single “Sequence Listing” in a single file and be submitted either:
  - (1) Electronically via the USPTO patent electronic filing system, where the file must not exceed 100 MB, and file compression is not permitted; or
  - (2) On a read-only optical disc(s), in compliance with [§ 1.52(e)](/cfr/37/1.52.md?p=e), where:
    - (i) A file that is not compressed must be contained on a single read-only optical disc;
    - (ii) The file may be compressed using WinZip®, 7-Zip, or Unix®/Linux® Zip;
    - (iii) A compressed file must not be self-extracting; and
    - (iv) A compressed ASCII plain text file that does not fit on a single read-only optical disc may be split into multiple file parts, in accordance with the target read-only optical disc size, and labeled in compliance with [§ 1.52(e)(5)(vi)](/cfr/37/1.52.md?p=e-5-vi).

## Notes

### Amendments

[86 FR 57051, Oct. 14, 2021]

### Source

Source: Sections 1.821 through 1.825 appear at 55 FR 18245, May 1, 1990, unless otherwise noted.

### Authority

Authority: 35 U.S.C. 2(b)(2), unless otherwise noted.

### Source

Source: 24 FR 10332, Dec. 22, 1959, unless otherwise noted.

### Amendments

[86 FR 57051, Oct. 14, 2021]